Review



pe cd127 monoclonal antibody (a7r34)  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Thermo Fisher pe cd127 monoclonal antibody (a7r34)
    Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − <t>CD127</t> + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
    Pe Cd127 Monoclonal Antibody (A7r34), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd127+(a7r34/anti+cd127/pmc12205599-20-0-6
    Average 90 stars, based on 1 article reviews
    pe cd127 monoclonal antibody (a7r34) - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s"

    Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s

    Journal: iScience

    doi: 10.1016/j.isci.2025.112800

    Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − CD127 + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
    Figure Legend Snippet: Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − CD127 + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .

    Techniques Used: Infection, Control, Injection, Adoptive Transfer Assay, Staining, Two Tailed Test

    IRF4 deficiency compromises MHC class Ⅱ expression and further restrains ILC-mediated apoptosis of effector CD4 + T cells both in vitro and in vivo (A) Violin plots visualizing the expression of MHC-class-Ⅱ-related signature genes. (B) FACS analysis and MFI of MHC class Ⅱ expression in ILC3s isolated from Irf4 f/f and Irf4 f/f Rorc cre mice ( n = 6). ILC3 subsets were gated as Lin − RORγt + and then CCR6 + NKp46 − , CCR6 − NKp46 + , or CCR6 − NKp46 − . The lineage cocktail included TCRγδ, CD3ε, CD19, CD5, CD11c, Gr-1, and Ter119. (C and D) Activated OT-Ⅱ CD4 + T cells were cultured ex vivo with purified ILC3s from the siLP of Irf4 f/f and Irf4 f/f Rorc cre mice in the presence or absence of Ova peptide or the anti-MHC class Ⅱ neutralizing antibody. (C) Quantification of OT-Ⅱ T cells recovery (%). (D) Quantification of Annexin-V + OT-Ⅱ T cells. (E and F) Naive CD4-positive T cells (gating as CD4 + CD25 − CD62L hi CD44 lo cells) were sorted from OT-Ⅱ mice and pre-activated overnight. After pre-activation, CD4 positive T cells were transplanted into recipient Irf4 f/f and Irf4 f/f Rorc cre mice along with OVA peptide administration every 2 days following transfer. Nine days later, mice were sacrificed for further analysis of OT-Ⅱ CD4 + T cells (gating as CD8 − CD4 + TCRβ + Vβ5 + ) transferred in the spleen, mLN, siLPL, and cLPL of recipient mice. (G) One hundred thousand intestinal ILC3s (Lin − CD127 + CD27 − KLRG1 − ) were sorted from Irf4 f/f or Irf4 f/f Rorc cre mice and transferred with 500,000 activated OT-ⅡCD4 + T cells into NCG mice, flowing by OVA peptide i.p. every 2 days. Nine days later, survived OT-ⅡCD4 + T cells were quantified. ( n = 2 APC, n = 5 transferred group). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments (B–G). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
    Figure Legend Snippet: IRF4 deficiency compromises MHC class Ⅱ expression and further restrains ILC-mediated apoptosis of effector CD4 + T cells both in vitro and in vivo (A) Violin plots visualizing the expression of MHC-class-Ⅱ-related signature genes. (B) FACS analysis and MFI of MHC class Ⅱ expression in ILC3s isolated from Irf4 f/f and Irf4 f/f Rorc cre mice ( n = 6). ILC3 subsets were gated as Lin − RORγt + and then CCR6 + NKp46 − , CCR6 − NKp46 + , or CCR6 − NKp46 − . The lineage cocktail included TCRγδ, CD3ε, CD19, CD5, CD11c, Gr-1, and Ter119. (C and D) Activated OT-Ⅱ CD4 + T cells were cultured ex vivo with purified ILC3s from the siLP of Irf4 f/f and Irf4 f/f Rorc cre mice in the presence or absence of Ova peptide or the anti-MHC class Ⅱ neutralizing antibody. (C) Quantification of OT-Ⅱ T cells recovery (%). (D) Quantification of Annexin-V + OT-Ⅱ T cells. (E and F) Naive CD4-positive T cells (gating as CD4 + CD25 − CD62L hi CD44 lo cells) were sorted from OT-Ⅱ mice and pre-activated overnight. After pre-activation, CD4 positive T cells were transplanted into recipient Irf4 f/f and Irf4 f/f Rorc cre mice along with OVA peptide administration every 2 days following transfer. Nine days later, mice were sacrificed for further analysis of OT-Ⅱ CD4 + T cells (gating as CD8 − CD4 + TCRβ + Vβ5 + ) transferred in the spleen, mLN, siLPL, and cLPL of recipient mice. (G) One hundred thousand intestinal ILC3s (Lin − CD127 + CD27 − KLRG1 − ) were sorted from Irf4 f/f or Irf4 f/f Rorc cre mice and transferred with 500,000 activated OT-ⅡCD4 + T cells into NCG mice, flowing by OVA peptide i.p. every 2 days. Nine days later, survived OT-ⅡCD4 + T cells were quantified. ( n = 2 APC, n = 5 transferred group). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments (B–G). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .

    Techniques Used: Expressing, In Vitro, In Vivo, Isolation, Cell Culture, Ex Vivo, Purification, Activation Assay, Two Tailed Test

    Cleavage under targets and tagmentation sequencing of IRF4 binding in intestinal ILC3 subsets (A) Heatmap showing the genome-wide distribution of IRF4-binding signals at peak centers in ILC3 subsets sorted from Irf4 f/f and Irf4 f/f Rorc cre mice by CUT&Tag. (B) Occupancy of IRF4 at all gene promoter regions (±5 kb of TSS). (C) Donut chart showing the percentages of IRF4 binding at exon regions, intron regions, or intergenic regions. (D) Venn plot displaying the overlap of the IRF4-regulated genes from pairwise comparisons of NKp46 + ILC3s, NKp46 − CCR6 − ILC3s, and CCR6 + ILC3s. (E) Gene set enrichment analysis (GSEA) of NKp46 + ILC3 signature gene sets enriched in shared IRF4-modified genes. (F and G) IGV visualizes the indicated gene locus containing ATAC-seq and IRF4-binding peaks in ILC3 subsets. IRF4 CUT&Tag sequencing data are from two independent replicates. (H–K) Rescue experiments. Retroviruses were generated by transfection of pMX-IRES-GFP plasmids containing the indicated genes into Plat-E cells using PolyJet. CLPs (Lin − CD127 + c-Kit int Sca-1 int Flt3 + ) were sorted from the bone marrow from Irf4 f/f and Irf4f/f Rorc cre mice and transfected with retroviral supernatants. Retrovirus-transfected CLPs were then collected and adoptively transferred into sublethally irradiated CD45.1 + wild-type recipient mice through intravenous tail vein injection. Transduced cells were transferred with CD45.1 + wild-type bone marrow cells to help the engraftment of the CLPs. After 2 weeks, recipient mice were sacrificed, and organs were collected for analysis. (H) Flow cytometry of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (upper). MHC class II expression of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (lower). (I) Cytokine production in siLP CD45.2 + GFP + ILC3s from the indicated recipient mice. (J) The percentages of the indicated subsets were compared. (K) The percentages of MHC class II + ILC3s, IL-17A + ILC3s, and IL-22 + ILC3s were compared. Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments. See also .
    Figure Legend Snippet: Cleavage under targets and tagmentation sequencing of IRF4 binding in intestinal ILC3 subsets (A) Heatmap showing the genome-wide distribution of IRF4-binding signals at peak centers in ILC3 subsets sorted from Irf4 f/f and Irf4 f/f Rorc cre mice by CUT&Tag. (B) Occupancy of IRF4 at all gene promoter regions (±5 kb of TSS). (C) Donut chart showing the percentages of IRF4 binding at exon regions, intron regions, or intergenic regions. (D) Venn plot displaying the overlap of the IRF4-regulated genes from pairwise comparisons of NKp46 + ILC3s, NKp46 − CCR6 − ILC3s, and CCR6 + ILC3s. (E) Gene set enrichment analysis (GSEA) of NKp46 + ILC3 signature gene sets enriched in shared IRF4-modified genes. (F and G) IGV visualizes the indicated gene locus containing ATAC-seq and IRF4-binding peaks in ILC3 subsets. IRF4 CUT&Tag sequencing data are from two independent replicates. (H–K) Rescue experiments. Retroviruses were generated by transfection of pMX-IRES-GFP plasmids containing the indicated genes into Plat-E cells using PolyJet. CLPs (Lin − CD127 + c-Kit int Sca-1 int Flt3 + ) were sorted from the bone marrow from Irf4 f/f and Irf4f/f Rorc cre mice and transfected with retroviral supernatants. Retrovirus-transfected CLPs were then collected and adoptively transferred into sublethally irradiated CD45.1 + wild-type recipient mice through intravenous tail vein injection. Transduced cells were transferred with CD45.1 + wild-type bone marrow cells to help the engraftment of the CLPs. After 2 weeks, recipient mice were sacrificed, and organs were collected for analysis. (H) Flow cytometry of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (upper). MHC class II expression of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (lower). (I) Cytokine production in siLP CD45.2 + GFP + ILC3s from the indicated recipient mice. (J) The percentages of the indicated subsets were compared. (K) The percentages of MHC class II + ILC3s, IL-17A + ILC3s, and IL-22 + ILC3s were compared. Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments. See also .

    Techniques Used: Sequencing, Binding Assay, Genome Wide, Modification, Generated, Transfection, Retroviral, Irradiation, Injection, Flow Cytometry, Isolation, Expressing, Two Tailed Test

    Related Articles

    Staining:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    Blocking Assay:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    Expressing:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    Infection:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    Injection:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    Ex Vivo:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    Adoptive Transfer Assay:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    MANN-WHITNEY:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    Software:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    Recombinant:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    Flow Cytometry:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    Fluorescence:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    Activation Assay:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.

    Comparison:

    Article Title: Eosinophils promote effector functions of lung group 2 innate lymphoid cells in allergic airway inflammation in mice.
    Article Snippet: F4/80 (BM8), Gr1 (RB6-8C5) CD3 (145-2C11), CD4 (GK1.5), CD8 (53- 6.7), B220 (RA3-6B2), CD19 (1D3), CD11b (M1/70) , CD11c (HL3), FεR1 (MAR-1), TER119 (Ter-119), NK1.1 (PK136), CD90.2 (53-2.1), ICOS (7E.17G9), CD127 (A7R34), CD45.2 (104), KLRG1 (2F1), CD25 (PC61.5), Ki-67 (So1A15), GATA-3 (TWAJ), CD206 (MR6F3), and IL-13 (eBio13A) were from eBioscience.

    Article Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection
    Article Snippet: To identify mouse ILCs, single-cell suspensions were stained with mAb for known lineages and with mAb discriminating ILC subsets. mAbs for known lineages included CD3 (17A2, Biolegend), CD4 (RM4-5, Biolegend), CD8a (53-6.7, Biolegend), TCRαβ (H57-597, Biolegend), TCRγδ, (GL3, Biolegend) CD11b (M1/70, Biolegend), CD11c (N418, Biolegend), F4/80 (BM8, Biolegend), Ly6G (1A8, Biolegend), TER119 (TER-119, Biolegend), FcεRIa (MAR-1, Biolegend), CD19 (1D3/CD19, Biolegend), B220 (RA3-6B2, Biolegend), and CD49b (DX5, Biolegend). mAbs discriminating ILC subsets included CD45.2 (104, BD), CD90.2 (30-H12, Biolegend), CD127 (A7R34, eBioscience), NK1.1 (PK136, BD Biosciences), IL-18Rα (P3TUNYA, eBioscience), ST2 (RMST2-2, eBioscience), CD226 (10E5, Biolegend), and CD49a (Ha31/8), NKp46 (29A1.4). mAbs for intracellular staining included GATA3 (L50-823, BD Biosciences), T-bet (4B10, eBiosciences), RORγt (Q31-378, BD Biosciences), TCF-1 (S33-966, BD), Arg1 (A1exF5, BD Biosciences), Ki-67 (SolA15, eBiosciences), and Eomes (Dan11mag, eBiosciences).

    Article Title: mTORC1 and mTORC2 differentially promote natural killer cell development
    Article Snippet: CD3 (17A2), NK1.1 (PK136), CD49b (DX5), CD27 (LG.7F9), CD11b (M1/70), KLRG1 (2F1), NCR1 (29A1.4), NKG2D (CX5), Ly49H (3D10), NKG2A/C/E (20d5), CD122 (5H4 or TM-b1), Ki-67 (SolA15), Eomes (Dan11mag), T-bet (4B10), CD127 (A7R34), CD244.2 (eBio244F4), CD107a (eBio1D4B), IFN-g (XMG1.2), Streptavidin-PE, Donkey anti-Rabbit second antibodies are from Thermo-Fisher Scientific (Waltham, MA); Ly49D (4E5), CD45.2 (104), CD132 (TUGm2), CD135 (A2F10), Biotin-Ccr7 (4B12) are from Biolegend (San Diego, CA); Ly49A (A1), Ly49G2 (4D11), Ly49C/I (5E6), p-STAT5Y694 (47) are from BD Pharmingen (San Jose, CA); Raptor (24C12), Rictor (53A2), p-AktS473 (D9E), p-rpS6S240/244 (D68F8), p-4E-BP1T37/46 Yang et al. eLife 2018;7:e35619.

    Article Title: A Combination of Anti-PD-L1 Treatment and Therapeutic Vaccination Facilitates Improved Retroviral Clearance via Reactivation of Highly Exhausted T Cells
    Article Snippet: The following monoclonal antibodies were used. αCD4 (clone RM4-5), αCD8 (clone 53-6.7), αCD80 (clone 16-10A1), αPD-1 (clone J43), and αCD43 (clone 1B11) were obtained from BD Biosciences Pharmingen. αGzmB (clone GB12) and αTOX (clone TXRX10) antibodies were purchased from Invitrogen. αFoxp3 (clone FJK-16s), Ki67 (clone SolA15), and CD127 (clone A7R34) antibodies were purchased from eBioscience. αIFN-y (clone XMG1.2), αTNF-α (clone MP6-XT22), αTCF1 (clone 7F11A10), and CXCR5 (clone L138D7) antibodies were obtained from BioLegend.

    Article Title: TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation
    Article Snippet: Single-cell suspensions were stained for 30 min at 4°C with combinations of the following antibodies, diluted in FcR block (CD16/CD32 clone 2.4G2, # 553142, RRID: AB_394657; BD Biosciences): CD45 (clone 30F11, # 557235, RRID: AB_396609; BD Biosciences and # 17-0451-82, RRID: AB_469392; eBioscience), SiglecF (clone E50-2440, # 552126, RRID: AB_394341; BD Biosciences), CD4 (clone GK1.5, # 11-0041-85, RRID: AB_464892; eBioscience), CD19 (clone eBio1D3, # 561740, RRID: AB_396681; BD Biosciences), CD45R (clone RA3-6B2, # 553088, RRID: AB_394618; BD biosciences), NK1.1 (clone PK136, # 11-5941-85, RRID: AB_465318; eBioscience and # 108739, RRID:AB_2562273; Biolegend), CD3 (clone 17A2, # 11-0032-82, RRID: AB_2572431; eBioscience and eF450 # 48-0032-82, RRID: AB_1272193; eBioscience), CD11b (clone M1/70, # 11-0112-85, RRID: AB_464936; eBioscience), Ter119 (clone Ter119, # 11-5921-85, RRID: AB_465311; eBioscience), Ly-6G/Ly6C (Gr-1, clone RB6-8C5, # 11-5931-81, RRID: AB_465313; eBioscience), FcεRIa (clone MAR-1, # 11-5898-85, RRID: AB_465308; eBioscience), CD11c (clone N418, # 11-0114-85, RRID: AB_464941; eBioscience and # 17-0114-82, RRID: AB_469346), CD90.2 (clone 53–2.1, # 561641, RRID: AB_10898013; BD Biosciences), Sca-1 (clone D7, # 17-5981-83, RRID: AB_469488; eBioscience), CD25 (clone PC61.5, # 48-0251-82, RRID: AB_10671550; eBioscience), CD127 (clone A7R34, # 12-1,271-82, RRID: AB_465844; eBioscience), KLRG1 (clone 2F1, # 17-5893-81, RRID: AB_469469; eBioscience), ICOS (clone 7E-17G9, # 12-9942-82, RRID: AB_466274; eBioscience), DR3 (clone 4C12, # 144405, RRID: AB_2561688; Biolegend), CD200 (clone OX-90, # 123807, RRID: AB_2275651; Biolegend), IL-33R (T1/ST2, clone DJ8, # 101001B, RRID: AB_947551; MD Biosciences), TL1A (clone Tandys1a, # 46-7911-82, RRID: AB_11217878; eBioscience), EpCAM (CD326, clone G8.8, # 118205, RRID: AB_1134176; BioLegend), CD31 (clone MEC13.3, # 102513; RRID: AB_493413; BioLegend), TCR beta (clone H57-597, # 48-5961-80, RRID:AB_11062012; eBioscience), and TCR gamma/delta (clone eBioGL3, # 48-5711-82, RRID:AB_2574071; eBioscience).

    Article Title: Lactate regulation of activation in CD8+ T cells
    Article Snippet: Fluorochrome-labelled antibodies against mouse antigens CD44 (clone IM7), CD8 (clone 53-6.7), CTLA-4 (clone UC10-4F10-11), LAG-3 (clone C9B7W), and PD-1 (clone J43) were purchased from BD Biosciences, CD27 (clone LG.3A10), CD28 (clone 37.51), CD62L (clone MEL-14), and ICOS (clone C398.4A) were purchased from BioLegend, and 4-1BB (clone 17B5), CD127 (clone A7R34), CD25 (clone PC61.5), Eomes (clone Dan11mag), T-bet (clone eBio4B10), and anti-human/mouse Granzyme B (clone GB12) was purchased from Thermo Fisher.

    Article Title: Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models.
    Article Snippet: Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses.. The presence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses.. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion.



    Similar Products

    3175006b  (fluidigm)
    94
    fluidigm 3175006b
    3175006b, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd127+(a7r34/Anti-Mouse+CD127%2FIL7Ra+(A7R34)-175Lu/pm41916320-680-132-130
    Average 94 stars, based on 1 article reviews
    3175006b - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    pe cd127 monoclonal antibody (a7r34)  (Thermo Fisher)
    90
    Thermo Fisher pe cd127 monoclonal antibody (a7r34)
    Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − <t>CD127</t> + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
    Pe Cd127 Monoclonal Antibody (A7r34), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd127+(a7r34/anti+cd127/pmc12205599-20-0-6
    Average 90 stars, based on 1 article reviews
    pe cd127 monoclonal antibody (a7r34) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    biotin anti-mouse cd127 (clone a7r34)  (Thermo Fisher)
    90
    Thermo Fisher biotin anti-mouse cd127 (clone a7r34)
    Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − <t>CD127</t> + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
    Biotin Anti Mouse Cd127 (Clone A7r34), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd127+(a7r34/anti+cd127/pmc12173640-7-0-7
    Average 90 stars, based on 1 article reviews
    biotin anti-mouse cd127 (clone a7r34) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    pe-cyanine7 anti-mouse cd127(clone a7r34)  (Thermo Fisher)
    90
    Thermo Fisher pe-cyanine7 anti-mouse cd127(clone a7r34)
    Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − <t>CD127</t> + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
    Pe Cyanine7 Anti Mouse Cd127(clone A7r34), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd127+(a7r34/anti+cd127/pm40478733-185-88-90
    Average 90 stars, based on 1 article reviews
    pe-cyanine7 anti-mouse cd127(clone a7r34) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    biotin anti-mouse cd127 antibody (clone a7r34)  (Sony)
    90
    Sony biotin anti-mouse cd127 antibody (clone a7r34)
    Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − <t>CD127</t> + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
    Biotin Anti Mouse Cd127 Antibody (Clone A7r34), supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd127+(a7r34/anti+mouse+f4+80+bm8++bv605/pm40402745-179-96-102
    Average 90 stars, based on 1 article reviews
    biotin anti-mouse cd127 antibody (clone a7r34) - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    anti-cd127 #46-1273-82 a7r34  (Thermo Fisher)
    90
    Thermo Fisher anti-cd127 #46-1273-82 a7r34
    Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − <t>CD127</t> + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
    Anti Cd127 #46 1273 82 A7r34, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd127+(a7r34/anti+cd127/pm40033120-530-7-76
    Average 90 stars, based on 1 article reviews
    anti-cd127 #46-1273-82 a7r34 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    rat anti-mouse cd127 monoclonal antibody (a7r34), efluortm 660  (Thermo Fisher)
    90
    Thermo Fisher rat anti-mouse cd127 monoclonal antibody (a7r34), efluortm 660
    Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − <t>CD127</t> + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
    Rat Anti Mouse Cd127 Monoclonal Antibody (A7r34), Efluortm 660, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd127+(a7r34/anti+cd127+apc/pmc11835792-291-21-23
    Average 90 stars, based on 1 article reviews
    rat anti-mouse cd127 monoclonal antibody (a7r34), efluortm 660 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    invivomab anti mouse il 7r cd127 a7r34 bio x cell be0065 invivoplus anti mouse cd8  (Bio X Cell)
    93
    Bio X Cell invivomab anti mouse il 7r cd127 a7r34 bio x cell be0065 invivoplus anti mouse cd8
    Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − <t>CD127</t> + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
    Invivomab Anti Mouse Il 7r Cd127 A7r34 Bio X Cell Be0065 Invivoplus Anti Mouse Cd8, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd127+(a7r34/InVivoMAb+anti-mouse+IL-7R%CE%B1/pmc11730321__41467_2025_55941_MOESM7_ESM-34-172-177
    Average 93 stars, based on 1 article reviews
    invivomab anti mouse il 7r cd127 a7r34 bio x cell be0065 invivoplus anti mouse cd8 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    cd127 (a7r34  (Thermo Fisher)
    90
    Thermo Fisher cd127 (a7r34
    Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − <t>CD127</t> + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
    Cd127 (A7r34, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd127+(a7r34/pm39792675-264-79-108
    Average 90 stars, based on 1 article reviews
    cd127 (a7r34 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    a-cd127-alexa fluor 700 a7r34  (Thermo Fisher)
    90
    Thermo Fisher a-cd127-alexa fluor 700 a7r34
    Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − <t>CD127</t> + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .
    A Cd127 Alexa Fluor 700 A7r34, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd127+(a7r34/pm39786996-392-39-44
    Average 90 stars, based on 1 article reviews
    a-cd127-alexa fluor 700 a7r34 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − CD127 + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .

    Journal: iScience

    Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s

    doi: 10.1016/j.isci.2025.112800

    Figure Lengend Snippet: Defective ILC3 function in IRF4-deficient mice drives C. rodentium and C. albicans infection in a cell-intrinsic manner (A–F) NCG mice were adoptively transferred with 80,000 intestinal ILC3s (Lin − CD127 + CD27 − KLRG1 − ) sorted from the small intestine of Irf4 f/f and Irf4 f/f Rorc cre mice or PBS as control after being treated with ABX for 1 week. ILC3s were stimulated with IL-23 and IL-1β for 30 min before injected into NCG mice through the tail vein. NCG mice were orally inoculated with C. rodentium 24 h after adoptive transfer. (A, B) Measurements (A) and statistical analysis (B) of the colon lengths from NCG recipients ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (C) CFUs in the feces of NCG recipients 9 days after infection ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (D) Changes in body weight were recorded at the indicated time points ( n = 4 control, n = 5 WT transferred, n = 7 KO transferred). (E) H&E staining of colon tissue sections. (F) Pathological score of colon histology. (G–L) Irf4 f/f or Irf4 f/f Rorc cre ILC3s were adoptively transferred into NCG mice with C. albicans infection. (G and H) Measurements (G) and statistical analysis (H) of the colon lengths from NCG recipients ( n = 2 control, n = 4 transferred). (I) Fecal fungal burden ( n = 3 control, n = 4 transferred). (J) Variations of body weight were shown each day ( n = 4). (K) Pathological score of colon histology. (L) Histological analysis of colonic tissues by H&E staining. The data are representative of at least two independent experiments (A–L). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .

    Article Snippet: PE CD127 Monoclonal Antibody (A7R34) , eBioscience , Cat# 12-1271-82; RRID: AB_465844.

    Techniques: Infection, Control, Injection, Adoptive Transfer Assay, Staining, Two Tailed Test

    IRF4 deficiency compromises MHC class Ⅱ expression and further restrains ILC-mediated apoptosis of effector CD4 + T cells both in vitro and in vivo (A) Violin plots visualizing the expression of MHC-class-Ⅱ-related signature genes. (B) FACS analysis and MFI of MHC class Ⅱ expression in ILC3s isolated from Irf4 f/f and Irf4 f/f Rorc cre mice ( n = 6). ILC3 subsets were gated as Lin − RORγt + and then CCR6 + NKp46 − , CCR6 − NKp46 + , or CCR6 − NKp46 − . The lineage cocktail included TCRγδ, CD3ε, CD19, CD5, CD11c, Gr-1, and Ter119. (C and D) Activated OT-Ⅱ CD4 + T cells were cultured ex vivo with purified ILC3s from the siLP of Irf4 f/f and Irf4 f/f Rorc cre mice in the presence or absence of Ova peptide or the anti-MHC class Ⅱ neutralizing antibody. (C) Quantification of OT-Ⅱ T cells recovery (%). (D) Quantification of Annexin-V + OT-Ⅱ T cells. (E and F) Naive CD4-positive T cells (gating as CD4 + CD25 − CD62L hi CD44 lo cells) were sorted from OT-Ⅱ mice and pre-activated overnight. After pre-activation, CD4 positive T cells were transplanted into recipient Irf4 f/f and Irf4 f/f Rorc cre mice along with OVA peptide administration every 2 days following transfer. Nine days later, mice were sacrificed for further analysis of OT-Ⅱ CD4 + T cells (gating as CD8 − CD4 + TCRβ + Vβ5 + ) transferred in the spleen, mLN, siLPL, and cLPL of recipient mice. (G) One hundred thousand intestinal ILC3s (Lin − CD127 + CD27 − KLRG1 − ) were sorted from Irf4 f/f or Irf4 f/f Rorc cre mice and transferred with 500,000 activated OT-ⅡCD4 + T cells into NCG mice, flowing by OVA peptide i.p. every 2 days. Nine days later, survived OT-ⅡCD4 + T cells were quantified. ( n = 2 APC, n = 5 transferred group). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments (B–G). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .

    Journal: iScience

    Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s

    doi: 10.1016/j.isci.2025.112800

    Figure Lengend Snippet: IRF4 deficiency compromises MHC class Ⅱ expression and further restrains ILC-mediated apoptosis of effector CD4 + T cells both in vitro and in vivo (A) Violin plots visualizing the expression of MHC-class-Ⅱ-related signature genes. (B) FACS analysis and MFI of MHC class Ⅱ expression in ILC3s isolated from Irf4 f/f and Irf4 f/f Rorc cre mice ( n = 6). ILC3 subsets were gated as Lin − RORγt + and then CCR6 + NKp46 − , CCR6 − NKp46 + , or CCR6 − NKp46 − . The lineage cocktail included TCRγδ, CD3ε, CD19, CD5, CD11c, Gr-1, and Ter119. (C and D) Activated OT-Ⅱ CD4 + T cells were cultured ex vivo with purified ILC3s from the siLP of Irf4 f/f and Irf4 f/f Rorc cre mice in the presence or absence of Ova peptide or the anti-MHC class Ⅱ neutralizing antibody. (C) Quantification of OT-Ⅱ T cells recovery (%). (D) Quantification of Annexin-V + OT-Ⅱ T cells. (E and F) Naive CD4-positive T cells (gating as CD4 + CD25 − CD62L hi CD44 lo cells) were sorted from OT-Ⅱ mice and pre-activated overnight. After pre-activation, CD4 positive T cells were transplanted into recipient Irf4 f/f and Irf4 f/f Rorc cre mice along with OVA peptide administration every 2 days following transfer. Nine days later, mice were sacrificed for further analysis of OT-Ⅱ CD4 + T cells (gating as CD8 − CD4 + TCRβ + Vβ5 + ) transferred in the spleen, mLN, siLPL, and cLPL of recipient mice. (G) One hundred thousand intestinal ILC3s (Lin − CD127 + CD27 − KLRG1 − ) were sorted from Irf4 f/f or Irf4 f/f Rorc cre mice and transferred with 500,000 activated OT-ⅡCD4 + T cells into NCG mice, flowing by OVA peptide i.p. every 2 days. Nine days later, survived OT-ⅡCD4 + T cells were quantified. ( n = 2 APC, n = 5 transferred group). Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments (B–G). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. See also .

    Article Snippet: PE CD127 Monoclonal Antibody (A7R34) , eBioscience , Cat# 12-1271-82; RRID: AB_465844.

    Techniques: Expressing, In Vitro, In Vivo, Isolation, Cell Culture, Ex Vivo, Purification, Activation Assay, Two Tailed Test

    Cleavage under targets and tagmentation sequencing of IRF4 binding in intestinal ILC3 subsets (A) Heatmap showing the genome-wide distribution of IRF4-binding signals at peak centers in ILC3 subsets sorted from Irf4 f/f and Irf4 f/f Rorc cre mice by CUT&Tag. (B) Occupancy of IRF4 at all gene promoter regions (±5 kb of TSS). (C) Donut chart showing the percentages of IRF4 binding at exon regions, intron regions, or intergenic regions. (D) Venn plot displaying the overlap of the IRF4-regulated genes from pairwise comparisons of NKp46 + ILC3s, NKp46 − CCR6 − ILC3s, and CCR6 + ILC3s. (E) Gene set enrichment analysis (GSEA) of NKp46 + ILC3 signature gene sets enriched in shared IRF4-modified genes. (F and G) IGV visualizes the indicated gene locus containing ATAC-seq and IRF4-binding peaks in ILC3 subsets. IRF4 CUT&Tag sequencing data are from two independent replicates. (H–K) Rescue experiments. Retroviruses were generated by transfection of pMX-IRES-GFP plasmids containing the indicated genes into Plat-E cells using PolyJet. CLPs (Lin − CD127 + c-Kit int Sca-1 int Flt3 + ) were sorted from the bone marrow from Irf4 f/f and Irf4f/f Rorc cre mice and transfected with retroviral supernatants. Retrovirus-transfected CLPs were then collected and adoptively transferred into sublethally irradiated CD45.1 + wild-type recipient mice through intravenous tail vein injection. Transduced cells were transferred with CD45.1 + wild-type bone marrow cells to help the engraftment of the CLPs. After 2 weeks, recipient mice were sacrificed, and organs were collected for analysis. (H) Flow cytometry of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (upper). MHC class II expression of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (lower). (I) Cytokine production in siLP CD45.2 + GFP + ILC3s from the indicated recipient mice. (J) The percentages of the indicated subsets were compared. (K) The percentages of MHC class II + ILC3s, IL-17A + ILC3s, and IL-22 + ILC3s were compared. Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments. See also .

    Journal: iScience

    Article Title: The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s

    doi: 10.1016/j.isci.2025.112800

    Figure Lengend Snippet: Cleavage under targets and tagmentation sequencing of IRF4 binding in intestinal ILC3 subsets (A) Heatmap showing the genome-wide distribution of IRF4-binding signals at peak centers in ILC3 subsets sorted from Irf4 f/f and Irf4 f/f Rorc cre mice by CUT&Tag. (B) Occupancy of IRF4 at all gene promoter regions (±5 kb of TSS). (C) Donut chart showing the percentages of IRF4 binding at exon regions, intron regions, or intergenic regions. (D) Venn plot displaying the overlap of the IRF4-regulated genes from pairwise comparisons of NKp46 + ILC3s, NKp46 − CCR6 − ILC3s, and CCR6 + ILC3s. (E) Gene set enrichment analysis (GSEA) of NKp46 + ILC3 signature gene sets enriched in shared IRF4-modified genes. (F and G) IGV visualizes the indicated gene locus containing ATAC-seq and IRF4-binding peaks in ILC3 subsets. IRF4 CUT&Tag sequencing data are from two independent replicates. (H–K) Rescue experiments. Retroviruses were generated by transfection of pMX-IRES-GFP plasmids containing the indicated genes into Plat-E cells using PolyJet. CLPs (Lin − CD127 + c-Kit int Sca-1 int Flt3 + ) were sorted from the bone marrow from Irf4 f/f and Irf4f/f Rorc cre mice and transfected with retroviral supernatants. Retrovirus-transfected CLPs were then collected and adoptively transferred into sublethally irradiated CD45.1 + wild-type recipient mice through intravenous tail vein injection. Transduced cells were transferred with CD45.1 + wild-type bone marrow cells to help the engraftment of the CLPs. After 2 weeks, recipient mice were sacrificed, and organs were collected for analysis. (H) Flow cytometry of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (upper). MHC class II expression of CD45.2 + GFP + ILC3 subsets isolated from the siLP in the indicated recipient mice (lower). (I) Cytokine production in siLP CD45.2 + GFP + ILC3s from the indicated recipient mice. (J) The percentages of the indicated subsets were compared. (K) The percentages of MHC class II + ILC3s, IL-17A + ILC3s, and IL-22 + ILC3s were compared. Bar graphs are presented as mean ± SEM. A two-tailed Student’s t test was performed for comparisons. The data are representative of at least three independent experiments. See also .

    Article Snippet: PE CD127 Monoclonal Antibody (A7R34) , eBioscience , Cat# 12-1271-82; RRID: AB_465844.

    Techniques: Sequencing, Binding Assay, Genome Wide, Modification, Generated, Transfection, Retroviral, Irradiation, Injection, Flow Cytometry, Isolation, Expressing, Two Tailed Test